rabbit polyclonal cd69 antibody  (Thermo Fisher)

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Assessing mCD69 expression in primary mouse T cells using flow cytometry and [89Zr]-DFO-H1.2F3 uptake. A, Scheme of CD69 antibody binding: H1.2F3 monoclonal anti-CD69 binds to mCD69 on the surface of activated primary mouse T cells and other immune cells. B, Scheme of CD69 imaging: CD69 can be used as a biomarker to distinguish immunologically active tumors of mice that respond to checkpoint blockade (CBP) therapy (responders) from the tumors of mice that do not respond to therapy (nonresponders). C, Ex vivo flow cytometry analysis of CD69 expression for primary mouse T cells treated with PMA/ionomycin or untreated primary mouse T cells. D, Ex vivo [89Zr]-DFO-H1.2F3 uptake for primary mouse T cells treated with 50 ng/mL PMA and 1 μg/mL ionomycin, untreated primary mouse T cells, and CT26 cells. Uptake was measured on γ-counter and normalized to percent injected dose per million cells (% ID/106 cells). Error bars, SD.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Expressing, Flow Cytometry, Binding Assay, Imaging, Biomarker Assay, Ex Vivo, Injection

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Validating CD69 expression in a CT26 syngeneic tumor immunotherapy model using ex vivo autoradiography and IHC. A, Representative autoradiography images of whole tumor sections from responders, nonresponders, and untreated control groups. B, 20x magnified histological and IHC images of CD69 expression (NovaRED) on tumors sections from responders, nonresponders, and untreated control groups. Scale bar, 100 μmol/L.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Expressing, Ex Vivo, Autoradiography

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Correlating CD69 IHC with immune lineage markers. A, Representative tumor sections from responder and nonresponder mice. Tumor sections were assayed for the CD69 activation marker using NovaRED, and for CD4, CD8, and CD45 lineage markers using DAB staining. Scale bar, 250 μmol/L. B to E, Quantification of the number of positively stained cells per high power field (HPF) for responder and nonresponder tumor sections, at 10x magnification. Scale bars, 250 μm. Two-tailed unpaired t test was used to compare groups., P < 0.05; **, P < 0.01; ***, P < 0.001;, P < 0.0001. n = 3 tumors per group with three measurements per HPF per tumor.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Activation Assay, Marker, Staining, Two Tailed Test

Journal: Mediators of Inflammation

Article Title: Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD

doi: 10.1155/2024/6263447

Figure Lengend Snippet: SCFAs alleviate inflammatory cell activities in gut ILC2 cells. (a) t-SNE feature plots revealing profiles of eight biomarkers. (b) Arg1, ST2, Thy1, KLRG1, IL-17RB, CCR9, and CD69 protein contents, as evidenced by Western blot analysis. (c) S1P, S1P2, S1P3, S1P4, and S1P5 transcript expressions, as evidenced by qRT-PCR. (d) Lung ILC2s mRNA expression in murine COPD model treated with butyrate. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.01 (relative to control mice).

Article Snippet: The membrane was blocked with 5% nonfat milk at room temperature for 2 hr and further incubated overnight with rabbit anti-arginase1 (anti-Arg1) (Sangon Biotech, Shanghai, China), Thy.1 monoclonal antibody (Proteintech Group, Inc, Wuhan, China), ST2 monoclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit antikiller cell lectin-like receptor G1 (KLRG1) (Abcam, Cambridge, MA, USA), rabbit anti-IL17RB antibody (bioss, Bejing, China), anti-CCR9 antibody (BosterBio, USA), rabbit anti-CD69 polyclonal antibody (absin, Shanghai, China), NFIL3 polyclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit anti-GATA3 polyclonal antibody (absin, Shanghai, China), histone-H3 polyclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit anti-acetyl-histone H3 monoclonal antibody (absin, Shanghai, China), or β -actin polyclonal antibody (Proteintech Group, Inc., Wuhan, China) at 4°C.

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Control